姓名：霍方俊
职称：教授
联系方式：huofj@sxu.edu.cn
介绍：
霍方俊，男，1978年12月生，博士，山西大学应用化学研究所教授，博士生导师，山西省“三晋英才”优秀青年人才。研究方向：化学生物学，从事有机小分子荧光探针的开发与生物传感研究工作。主持国家自然科学基金3项、山西省自然科学基金3项，山西省留学人员项目2项，获得授权发明专利13项，在国内外发表SCI论文150余篇，获得山西省自然科学一等奖1项、三等奖1项。
学习与工作经历：
2017.12 –至今, 山西大学, 应用化学研究所, 教授
2016.08 – 2017.11, 山西大学, 应用化学研究所, 副教授
2014.09 – 2016.07, 美国密苏里大学, 化学系, 访问学者
2009.10 – 2014.08, 山西大学, 应用化学研究所, 副教授
2007.07 – 2009.09, 山西大学, 应用化学研究所, 讲师
2004.09 – 2007.07, 山西大学, 分子科学研究所, 博士
2001.09 – 2004.07, 山西大学, 化学化工学院, 硕士
1996.09 – 2000.07, 山西大学, 化学化工学院, 学士
研究方向：
荧光探针与生物传感
主持科研项目：
1. 国家自然科学基金（面上项目），去甲肾上腺素快速、特异性荧光探针构建及其抑郁模型原位成像（22377071），50万，2024.01–2027.12， 在研；
2. 国家自然科学基金（联合基金项目），构建关节软骨浅表层损伤分子机制的荧光分子成像试剂研究（U23A6009），73万，2024.01–2027.12，在研；
3. 国家自然科学基金（青年项目），基于"硫醇-色烯"关环反应的可再生化学计量器属于重金离子检测和生物成像的研究（21102086），25万，2012.01–2014.12，已结题；
4. 山西省留学人员科技活动择优资助项目，基于层叠式级联反应的神经递质荧光检测和神经成像（2024001），12万，2024.06–2027.5, 在研；
5. 山西省自然科学基金，去甲肾上腺素高选择性、快速响应荧光探针合成及成像（202203021221009），10万，2023.01–2025.12，在研；
6. 山西省省筹资金资助回国留学人员项目，细胞器二氧化硫精准荧光成像及生理、病理机制研究（2022-002），6万，2022.08–2025.08，在研；
7. 山西省自然科学基金，光激活型多反应位点/癌细胞靶向二氧化硫精准荧光检测（201901D111015），5万，2019.09 –2022.09，已结题；
8. 山西省自然科学基金，基于点击反应的硫醇小分子光学探针的研究（2012021009-4），3万，2012.01–2014.12，已结题。
代表性论文：
[1] Waqas Pervez, Laraib, Caixia Yin*, Fangjun Huo*. Homocysteine fluorescent probes: Sensing mechanisms and biological applications, Coordination Chemistry Reviews, 2025, 522, 216202.
[2] Xinyu Li, Junping Wang, Fangjun Huo*, Caixia Yin*. Organic small molecule fluorescent probes based cascade reaction: Design strategies, biomedical applications and prospects, Coordination Chemistry Reviews, 2024, 506, 215713.
[3] Huming Yan, Yuting Wang, Fangjun Huo*, Caixia Yin*. Fast-specific fluorescent probes to visualize norepinephrine signaling pathways and its flux in the epileptic mice brain, Journal of the American Chemical Society, 2023, 145, 3229-3237.
[4] Yuting Wang, Huming Yan, Yongkang Yue, Yongbin Zhang, Fangjun Huo*, Fangqin Cheng*, Caixia Yin*. GSH and H2O2 dynamic correlation in the ferroptosis pathways revealed by engineered probe in tumor and kidney injury, Chemical Engineering Journal, 2023, 464, 142496.
[5] Hongjun Song, Weijie Zhang, Ya Zhang, Caixia Yin*, Fangjun Huo*. Viscosity activated NIR fluorescent probe for visualizing mitochondrial viscosity dynamic and fatty liver mice, Chemical Engineering Journal, 2022, 445, 136448.
[6] Yongfei Huang, Yongbin Zhang, Fangjun Huo*, Jianbin Chao, Caixia Yin*. A dual-targeted organelles SO2 specific probe for bioimaging in related diseases and food analysis, Chemical Engineering Journal, 2022, 433, 133750.
[7] Na Zhou, Caixia Yin*, Yongkang Yue, Yongbin Zhang, Fangqin Cheng, Fangjun Huo*. NIR fluorescent probe tracing norepinephrine exocytosis and depression occurrence at the cellular level, Chemical Communication, 2022, 58, 2999-3002.
[8] Yingchun Wu, Caixia Yin*, Weijie Zhang, Yongbin Zhang, Fangjun Huo*. Mitochondrial-targeting near-infrared fluorescent probe for visualizing viscosity in drug-induced cells and a fatty liver mouse model, Analytical Chemistry, 2022, 94, 5069-5074.
[9] Huming Yan#, Fangjun Huo#, Yongkang Yue, Jianbin Chao, Caixia Yin*. Rapid reaction, slow dissociation aggregation, and synergetic multicolor emission for imaging the restriction and regulation of biosynthesis of Cys and GSH, Journal of the American Chemical Society, 2021, 143, 318-325.
[10] Weijie Zhang#, Fangjun Huo#, Yongkang Yue, Yongbin Zhang, Jianbin Chao, Fangqin Cheng, Caixia Yin*. Heat stroke in cell tissues related to sulfur dioxide level is precisely monitored by light-controlled fluorescent probes, Journal of the American Chemical Society, 2020, 142, 3262-3268.
[11] Weijie Zhang#, Fangjun Huo#, Fangqin Cheng, Caixia Yin*. Employing an ICT-FRET integration platform for the real-time tracking of SO2 metabolism in cancer cells and tumor models, Journal of the American Chemical Society, 2020, 142, 6324-6331.
[12] Fangjun Huo*, Qing Wu, Jin Kang, Yongbin, Zhang, Caixia Yin*. A specific fluorescent probe for zinc ion based on thymolphthalein and its application in living cells, Sensors and Actuators B: Chemical, 2018, 262, 263-269.
[13] Fangjun Huo*, Yaqiong Zhang, Peng Ning, Xiangming Meng, Caixia Yin*. A novel isophorone-based red-emitting fluorescent probe for selective detection of sulfide anions in water for in vivo imaging, Journal of Materials Chemistry B, 2017, 5, 2798-2803.
[14] Yongkang Yue, Fangjun Huo, Peng Ning, Yongbin Zhang, Jianbin Chao, Xiangming Meng*, Caixia Yin*. Dual-site fluorescent probe for visualizing the metabolism of cys in living cells, Journal of the American Chemical Society, 2017, 139, 3181-3185.
[15] Fangjun Huo, Jin Kang, Caixia Yin*, Jianbin Chao, Yongbin zhang. Highly selective fluorescent and colorimetric probe for live-cell monitoring of sulphide based on bioorthogonalreaction, Scientific Reports, 2015, 5, 8969.
[16] Fangjun Huo*, Jin Kang, Caixia Yin*, Jianbin Chao, Yongbin Zhang. A turn on fluorescent sensor for cyanide based on ICT off in aqueous and its application for bioimaging, Sensors and Actuators B: Chemical, 2015, 215, 93-98.
[17] Fangjun Huo*, Jin Kang, Caixia Yin*, Jianbin Chao, Yongbin Zhang. A turn-on green fluorescent thiol probe based on the 1,2-additionreaction and its application for bioimaging, Sensors and Actuators B: Chemical, 2015, 207, 139-143.
[18] Caixia Yin, Jingjing Zhang, Ramon Martinez-Manez*, Fangjun Huo*, Yutao Yang, Haigang Lv, Sidian Li*. Thiol-addition reactions and their applications in thiol recognition, Chemical Society Reviews, 2013, 42(14), 6032-6059.
[19] Fangjun Huo, Yutao Yang, Caixia Yin*, Yueyin Chu, Jianbin Chao, Yongbin Zhang, Jingjing Zhang, Sidian Li, Haigang Lv, Anmin Zheng*, Diansheng Liu*. Combined spectral experiment and theoretical calculation to study the chemosensors of copper and their applications in anion bioimaging, Sensors and Actuators B: Chemical, 2013, 177, 1189-1197.
[20] Fangjun Huo*, Long Wang, Caixia Yin*, Yutao Yang, Hongbo Tong, Jianbin Chao, Yongbin zhang. The synthesis, characterization of three isomers ofrhodamine- derivative and their application in copper (II) ion recognition, Sensors and Actuators B: Chemical, 2013, 188, 735-740.
[21] Fangjun Huo, Caixia Yin*, Yutao Yang, Jing Su, Jianbin Chao, Diansheng Liu. Ultraviolet-visible light (Uv−vis)-reversible but fluorescence-irreversible chemosensor for copper in water and its application in living cells, Analytical Chemistry, 2012, 84, 2219-2223.
[22] Fangjun Huo, Jingjing Zhang, Yutao Yang, Jianbin Chao, Caixia Yin*, Yongbin Zhang, Tinggui Chen. A fluorescein-based highly specific colorimetric and fluorescent probe for hypochlorites in aqueous solution and its application in tap water, Sensors and Actuators B: Chemical, 2012, 166-67, 44-49.
[23] Fangjun Huo, Yutao Yang, Jing Su, Yuanqiang Sun, Caixia Yin*, Xuxiu Yan. Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma, Analyst, 2011, 136(9), 1892-1897.
[24] Fangjun Huo, Yuanqiang Sun, Jing Su, Yutao Yang, Caixia Yin*, Jianbin Chao. Chromene "lock", thiol "key", and mercury(ii) ion "hand": a single molecular machine recognition system, Organic Letters, 2010, 12(21), 4756-4759.
[25] Fangjun Huo, Yuanqiang Sun, Jing Su, Jianbin Chao, Haijuan Zhi, Caixia Yin*. Colorimetric detection of thiols using a chromene molecule, Organic Letters, 2009, 11(21), 4918-4921.
授权专利：
1. 霍方俊，宋红军，阴彩霞，一种苯并吡喃衍生物BP-Ⅲ及其合成方法和应用，国家发明专利，ZL202210155434.3，授权日期：2024.05.28；
2. 霍方俊，吴迎春，阴彩霞，一种螺吡喃-冠醚衍生物SP-CE及其合成方法和应用，国家发明专利，ZL202110146324.6，授权日期：2022.09.20；
3. 霍方俊，武青，阴彩霞，一种异氟尔酮硝基的衍生物及其合成方法和应用, 国家发明专利，ZL201811324832.3，授权日期：2021.5.14；
4. 霍方俊，周娜，阴彩霞，一种异恶唑衍生物及其合成方法和检测硫化氢的应用，国家发明专利，ZL201811324214.9，授权日期：2020.4.17；
5. 霍方俊，阴彩霞，一种CN-检测试剂及其合成方法和应用, 国家发明专利，ZL201710003440.6，授权日期：2018.10.16；
6. 霍方俊，阴彩霞，一种1,2-苯并异噻唑-3-酮的制备方法，国家发明专利，ZL201610255264.0，授权日期：2018.1.2；
7. 霍方俊，阴彩霞，一种试剂及其在硫醇检测中的应用，国家发明专利，ZL201410194221.7，授权日期：2016.2.10；
8. 霍方俊，阴彩霞，一种检测硫醇的试剂和方法，国家发明专利，ZL201310488140.3，授权日期：2015.7.29；
9. 霍方俊，阴彩霞，一种试剂及其在硫化氢检测中的应用，国家发明专利，ZL201410194129.0，授权日期：2015.6.3；
10. 霍方俊，阴彩霞，杨瑜涛，张改清，一种检测同型半胱氨酸的试剂和方法，国家发明专利，ZL201110191582.2，授权日期：2013.1.23；
11. 霍方俊，刘艳玲，阴彩霞，孙远强，快速检测水溶液中无机磷的方法，国家发明专利，ZL200910073643.8，授权日期：2011.1.5；
12. 霍方俊，阴彩霞，孙远强，快速检测水溶液中半胱氨酸的方法，国家发明专利，ZL200810055453.9，授权日期：2010.6.23；
13. 霍方俊，阴彩霞，杨频，一种基于胍环的核算水解切割试剂，国家发明专利，ZL200610048393.9，授权日期：2008.7.30。